|
KMID : 1025520040460050729
|
|
Journal of Animal Science and Technology
2004 Volume.46 No. 5 p.729 ~ p.734
|
|
|
Effect of Sequence Variation in Bovine Mitochondrial DNA D-loop Region on Milk Production for Hanwoo
|
|
Kong H. S.
Oh Jea-Don
Lim H. Y.
Lee Hak-Kyo
Jeon Gwang-Joo
Yoon Du-Hak
Jeon G. J.
Choi J. G.
Choy Yun-Ho
Cho Byung-Wook
|
|
|
Abstract
|
|
|
|
This study was performed to analyze the sequence variations of mtDNA D-loop and their effects on milk in Hanwoo(Korean cattle). The resulting sequences were compared with previously published sequences for other cattle breeds (GenBank J01394). The Polymerase Chain Reaction was performed to amplify a total of 964 bp between nucleotide 15758 and 383 within D-loop region of mtDNA using specific primers. Twenty polymorphic sites by nucleotide substitution were found in mtDNA D-loop region of Hanwoo. The frequencies of positions at 8, 169, 16042, 16051, 16057, 16093, 16119, 16122, 16209, 16255 and 16302 nt with high levels of sequence polymorphism were 0.150, 0.950, 0.085, 0.138, 0.106, 0.085, 0.138, 0.212, 0.085, 0.148 and 0.180, respectively. The substitution effect at 16119(p＜0.1) and 16185(p＜0.05) nt was found significant on milk production. Polymorphism of mtDNA sequence in D-loop region could be useful for the analysis of cytoplasmic genetic variation and associations with the other economically important traits and maternal lineage analysis in Hanwoo.
|
|
|
KEYWORD
|
|
|
mtDNA, D-loop region, Sequence variation, Hanwoo
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|